Key features and details
- Suitable for: Blocking
Product nameHuman Histone H4 (mono methyl K20) peptide
See all Histone H4 proteins and peptides
Modificationsmono methyl K20
Our Abpromise guarantee covers the use of ab17043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Information available upon request.
FunctionCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Sequence similaritiesBelongs to the histone H4 family.
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
All lanes : Anti-Histone H4 (mono methyl K20) antibody [mAbcam 16974] (ab16974) at 10 μg/ml
Lane 1 : HeLa Nuclear lysate
Lane 2 : HeLa Nuclear lysate with Human Histone H4 peptide (ab2622) at 0.2 μg/ml
Lane 3 : HeLa Nuclear lysate with
Human Histone H4 (mono methyl K20) peptide (ab17043) at 0.2 μg/ml
Lane 4 : HeLa Nuclear lysate with
Human Histone H4 (di methyl K20) peptide (ab14964) at 0.2 μg/ml
Lane 5 : HeLa Nuclear lysate with
Human Histone H4 (tri methyl K20) peptide (ab17567) at 0.2 μg/ml
Lysates/proteins at 20 μg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/10000 dilution
Performed under reducing conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab17043 has been referenced in 5 publications.
- Wells MB et al. Caenorhabditis elegans dosage compensation regulates histone H4 chromatin state on X chromosomes. Mol Cell Biol 32:1710-9 (2012). PubMed: 22393255
- FitzGerald J et al. Regulation of the DNA damage response and gene expression by the Dot1L histone methyltransferase and the 53Bp1 tumour suppressor. PLoS One 6:e14714 (2011). Blocking . PubMed: 21383990
- Edwards CR et al. Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing. Biochemistry 50:10473-83 (2011). PubMed: 21985125
- Wang Y et al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). PubMed: 19250904
- Heit R et al. G2 histone methylation is required for the proper segregation of chromosomes. J Cell Sci 122:2957-68 (2009). PubMed: 19638412